How to Make a GMO Plant

From hacking bacteria to blasting your way into the heart of the cell, in this episode we explore the ways to get a transgene into a plant cell and go from there to a whole genetically engineered plant. 

Key references

Herrera-Estrella L. My journey into the birth of plant transgenesis and its impact on modern plant biology. Plant Biotechnol J. 2020

Chimeric Genes as Dominant Selectable Markers in Plant Cells. Herrera-Estrella et al., 1983, the EMBO Journal. - First publication of delivering a recombinant T-DNA plasmid into plant cells.

History of Plant Tissue Culture. Trevor A Thorpe. University of Calgary

Current Advancement and Future Prospects in Simplified Transformation Based Plant Genome Editing. Han et al., Plants (Basel). 2025 - Review of plant transformation techniques. 

Deep Dive into Plant Transformation: Protoplast Mediated, Biolistic, Agrobacterium Mediated Gene Transfer. Dr Adriana Gallego. GoldBio.  - Article about different plant transformation methods.